Coding
Part:BBa_K1916019:Design
Designed by: Jonathan Chen Group: iGEM16_UC_Davis (2016-10-12)
CBCR8 + intein-CBD tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1084
Illegal AgeI site found at 1174 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
Source
Metagenomic data mining (JGI)